Integrated Mapping Analysis of the Werner Syndrome Region of Chromosome 8
Identifieur interne : 000962 ( Main/Exploration ); précédent : 000961; suivant : 000963Integrated Mapping Analysis of the Werner Syndrome Region of Chromosome 8
Auteurs : Junko Oshima [Canada] ; Chang-En Yu [Canada] ; Michael Boehnke [Canada] ; James L. Weber [Canada] ; Susanne Edelhoff [Canada] ; Michael J. Wagner [Canada] ; Dan E. Wells [Canada] ; Stephen Wood [Canada] ; Christine M. Disteche [Canada] ; George M. Martin [Canada] ; Gerard D. Schellenberg [Canada]Source :
- Genomics [ 0888-7543 ] ; 1994.
Abstract
Abstract: The Werner syndrome locus (WRN) is located at 8p11-p12. To facilitate eventual cloning of the WRN gene, a 10,000-rad radiation-reduced hybrid (RH) cell panel was generated to map genetic markers, sequence-tagged sites (STSs), and genes in this region. A hamster cell line carrying an intact human chromosome 8 was fused with another hamster cell line. Two sets of hybrid cell panels from 2 separate fusions were generated; each panel consisted of 50 independent clones; 33 and 34 cell lines from the 2 fusions retained human chromosome material as determined by inter-Alu PCR. The combined panel was genotyped for 52 markers spanning the entire chromosome, including 10 genes, 29 anonymous polymorphic loci, and 13 STSs. Seventeen of these markers have not been previously described. Markers near the centromere were retained at a higher frequency than more distal markers. Fluorescence in situ hybridization was also used to localize and order a subset of the markers. A RH map of the WRN region was constructed using a maximum likelihood method, giving the following most likely order: D8S131 - D8S339 (GSR) - D8S124 - D8S278 - D8S259 (D8S71) - D8S283- D8S87 - D8S105 - D8S135 (FGFR1) D8S135PB-D8S255-ANK1. A genetic map of 15 short tandem repeat polymorphic loci in the WRN region was also constructed. The marker orders from the genetic and RH maps were consistent. In addition, an integrated map of 24 loci in the WRN region was generated using information from both genetic and RH mapping methods. A 1000:1 framework map for 6 loci (LPL-D8S136-D8S137-D8S87-FGFR1-ANK1) was determined by genetic mapping, and the resulting locus order was fixed during analysis of the RH genotype data. The resulting integrated map contained more markers than could confidently be ordered by either genetic or RH mapping alone.
Url:
DOI: 10.1006/geno.1994.1464
Affiliations:
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Weber</name></author><author><name sortKey="Edelhoff, Susanne" sort="Edelhoff, Susanne" uniqKey="Edelhoff S" first="Susanne" last="Edelhoff">Susanne Edelhoff</name></author><author><name sortKey="Wagner, Michael J" sort="Wagner, Michael J" uniqKey="Wagner M" first="Michael J." last="Wagner">Michael J. Wagner</name></author><author><name sortKey="Wells, Dan E" sort="Wells, Dan E" uniqKey="Wells D" first="Dan E." last="Wells">Dan E. Wells</name></author><author><name sortKey="Wood, Stephen" sort="Wood, Stephen" uniqKey="Wood S" first="Stephen" last="Wood">Stephen Wood</name></author><author><name sortKey="Disteche, Christine M" sort="Disteche, Christine M" uniqKey="Disteche C" first="Christine M." last="Disteche">Christine M. Disteche</name></author><author><name sortKey="Martin, George M" sort="Martin, George M" uniqKey="Martin G" first="George M." last="Martin">George M. 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Weber</name><affiliation wicri:level="4"><country>Canada</country><wicri:regionArea>Department of Pathology and the Division of Neurology, University of Washington, Seattle, Washington 98195; Department of Biostatistics, University of Michigan, Ann Arbor, Michigan 48109-2029; Marshfield Medical Research Foundation, Marshfield, Wisconsin 54449; Department of Biology and Institute for Molecular Biology, University of Houston, Texas 77204-5513; Department of Medical Genetics, University of British Columbia, Vancouver, British Columbia</wicri:regionArea><orgName type="university">Université de Washington</orgName><placeName><settlement type="city">Seattle</settlement><region type="state">Washington (État)</region></placeName></affiliation></author><author><name sortKey="Edelhoff, Susanne" sort="Edelhoff, Susanne" uniqKey="Edelhoff S" first="Susanne" last="Edelhoff">Susanne Edelhoff</name><affiliation wicri:level="4"><country>Canada</country><wicri:regionArea>Department of Pathology and the Division of Neurology, University of Washington, Seattle, Washington 98195; Department of Biostatistics, University of Michigan, Ann Arbor, Michigan 48109-2029; Marshfield Medical Research Foundation, Marshfield, Wisconsin 54449; Department of Biology and Institute for Molecular Biology, University of Houston, Texas 77204-5513; Department of Medical Genetics, University of British Columbia, Vancouver, British Columbia</wicri:regionArea><orgName type="university">Université de Washington</orgName><placeName><settlement type="city">Seattle</settlement><region type="state">Washington (État)</region></placeName></affiliation></author><author><name sortKey="Wagner, Michael J" sort="Wagner, Michael J" uniqKey="Wagner M" first="Michael J." last="Wagner">Michael J. Wagner</name><affiliation wicri:level="4"><country>Canada</country><wicri:regionArea>Department of Pathology and the Division of Neurology, University of Washington, Seattle, Washington 98195; Department of Biostatistics, University of Michigan, Ann Arbor, Michigan 48109-2029; Marshfield Medical Research Foundation, Marshfield, Wisconsin 54449; Department of Biology and Institute for Molecular Biology, University of Houston, Texas 77204-5513; Department of Medical Genetics, University of British Columbia, Vancouver, British Columbia</wicri:regionArea><orgName type="university">Université de Washington</orgName><placeName><settlement type="city">Seattle</settlement><region type="state">Washington (État)</region></placeName></affiliation></author><author><name sortKey="Wells, Dan E" sort="Wells, Dan E" uniqKey="Wells D" first="Dan E." last="Wells">Dan E. Wells</name><affiliation wicri:level="4"><country>Canada</country><wicri:regionArea>Department of Pathology and the Division of Neurology, University of Washington, Seattle, Washington 98195; Department of Biostatistics, University of Michigan, Ann Arbor, Michigan 48109-2029; Marshfield Medical Research Foundation, Marshfield, Wisconsin 54449; Department of Biology and Institute for Molecular Biology, University of Houston, Texas 77204-5513; Department of Medical Genetics, University of British Columbia, Vancouver, British Columbia</wicri:regionArea><orgName type="university">Université de Washington</orgName><placeName><settlement type="city">Seattle</settlement><region type="state">Washington (État)</region></placeName></affiliation></author><author><name sortKey="Wood, Stephen" sort="Wood, Stephen" uniqKey="Wood S" first="Stephen" last="Wood">Stephen Wood</name><affiliation wicri:level="4"><country>Canada</country><wicri:regionArea>Department of Pathology and the Division of Neurology, University of Washington, Seattle, Washington 98195; Department of Biostatistics, University of Michigan, Ann Arbor, Michigan 48109-2029; Marshfield Medical Research Foundation, Marshfield, Wisconsin 54449; Department of Biology and Institute for Molecular Biology, University of Houston, Texas 77204-5513; Department of Medical Genetics, University of British Columbia, Vancouver, British Columbia</wicri:regionArea><orgName type="university">Université de Washington</orgName><placeName><settlement type="city">Seattle</settlement><region type="state">Washington (État)</region></placeName></affiliation></author><author><name sortKey="Disteche, Christine M" sort="Disteche, Christine M" uniqKey="Disteche C" first="Christine M." last="Disteche">Christine M. Disteche</name><affiliation wicri:level="4"><country>Canada</country><wicri:regionArea>Department of Pathology and the Division of Neurology, University of Washington, Seattle, Washington 98195; Department of Biostatistics, University of Michigan, Ann Arbor, Michigan 48109-2029; Marshfield Medical Research Foundation, Marshfield, Wisconsin 54449; Department of Biology and Institute for Molecular Biology, University of Houston, Texas 77204-5513; Department of Medical Genetics, University of British Columbia, Vancouver, British Columbia</wicri:regionArea><orgName type="university">Université de Washington</orgName><placeName><settlement type="city">Seattle</settlement><region type="state">Washington (État)</region></placeName></affiliation></author><author><name sortKey="Martin, George M" sort="Martin, George M" uniqKey="Martin G" first="George M." last="Martin">George M. Martin</name><affiliation wicri:level="4"><country>Canada</country><wicri:regionArea>Department of Pathology and the Division of Neurology, University of Washington, Seattle, Washington 98195; Department of Biostatistics, University of Michigan, Ann Arbor, Michigan 48109-2029; Marshfield Medical Research Foundation, Marshfield, Wisconsin 54449; Department of Biology and Institute for Molecular Biology, University of Houston, Texas 77204-5513; Department of Medical Genetics, University of British Columbia, Vancouver, British Columbia</wicri:regionArea><orgName type="university">Université de Washington</orgName><placeName><settlement type="city">Seattle</settlement><region type="state">Washington (État)</region></placeName></affiliation></author><author><name sortKey="Schellenberg, Gerard D" sort="Schellenberg, Gerard D" uniqKey="Schellenberg G" first="Gerard D." last="Schellenberg">Gerard D. Schellenberg</name><affiliation wicri:level="4"><country>Canada</country><wicri:regionArea>Department of Pathology and the Division of Neurology, University of Washington, Seattle, Washington 98195; Department of Biostatistics, University of Michigan, Ann Arbor, Michigan 48109-2029; Marshfield Medical Research Foundation, Marshfield, Wisconsin 54449; Department of Biology and Institute for Molecular Biology, University of Houston, Texas 77204-5513; Department of Medical Genetics, University of British Columbia, Vancouver, British Columbia</wicri:regionArea><orgName type="university">Université de Washington</orgName><placeName><settlement type="city">Seattle</settlement><region type="state">Washington (État)</region></placeName></affiliation></author></analytic><monogr></monogr><series><title level="j">Genomics</title><title level="j" type="abbrev">YGENO</title><idno type="ISSN">0888-7543</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1994">1994</date><biblScope unit="volume">23</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="100">100</biblScope><biblScope unit="page" to="113">113</biblScope></imprint><idno type="ISSN">0888-7543</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0888-7543</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: The Werner syndrome locus (WRN) is located at 8p11-p12. To facilitate eventual cloning of the WRN gene, a 10,000-rad radiation-reduced hybrid (RH) cell panel was generated to map genetic markers, sequence-tagged sites (STSs), and genes in this region. A hamster cell line carrying an intact human chromosome 8 was fused with another hamster cell line. Two sets of hybrid cell panels from 2 separate fusions were generated; each panel consisted of 50 independent clones; 33 and 34 cell lines from the 2 fusions retained human chromosome material as determined by inter-Alu PCR. The combined panel was genotyped for 52 markers spanning the entire chromosome, including 10 genes, 29 anonymous polymorphic loci, and 13 STSs. Seventeen of these markers have not been previously described. Markers near the centromere were retained at a higher frequency than more distal markers. Fluorescence in situ hybridization was also used to localize and order a subset of the markers. A RH map of the WRN region was constructed using a maximum likelihood method, giving the following most likely order: D8S131 - D8S339 (GSR) - D8S124 - D8S278 - D8S259 (D8S71) - D8S283- D8S87 - D8S105 - D8S135 (FGFR1) D8S135PB-D8S255-ANK1. A genetic map of 15 short tandem repeat polymorphic loci in the WRN region was also constructed. The marker orders from the genetic and RH maps were consistent. In addition, an integrated map of 24 loci in the WRN region was generated using information from both genetic and RH mapping methods. A 1000:1 framework map for 6 loci (LPL-D8S136-D8S137-D8S87-FGFR1-ANK1) was determined by genetic mapping, and the resulting locus order was fixed during analysis of the RH genotype data. The resulting integrated map contained more markers than could confidently be ordered by either genetic or RH mapping alone.</div></front></TEI><affiliations><list><country><li>Canada</li></country><region><li>Washington (État)</li></region><settlement><li>Seattle</li></settlement><orgName><li>Université de Washington</li></orgName></list><tree><country name="Canada"><region name="Washington (État)"><name sortKey="Oshima, Junko" sort="Oshima, Junko" uniqKey="Oshima J" first="Junko" last="Oshima">Junko Oshima</name></region><name sortKey="Boehnke, Michael" sort="Boehnke, Michael" uniqKey="Boehnke M" first="Michael" last="Boehnke">Michael Boehnke</name><name sortKey="Disteche, Christine M" sort="Disteche, Christine M" uniqKey="Disteche C" first="Christine M." last="Disteche">Christine M. Disteche</name><name sortKey="Edelhoff, Susanne" sort="Edelhoff, Susanne" uniqKey="Edelhoff S" first="Susanne" last="Edelhoff">Susanne Edelhoff</name><name sortKey="Martin, George M" sort="Martin, George M" uniqKey="Martin G" first="George M." last="Martin">George M. Martin</name><name sortKey="Schellenberg, Gerard D" sort="Schellenberg, Gerard D" uniqKey="Schellenberg G" first="Gerard D." last="Schellenberg">Gerard D. Schellenberg</name><name sortKey="Wagner, Michael J" sort="Wagner, Michael J" uniqKey="Wagner M" first="Michael J." last="Wagner">Michael J. Wagner</name><name sortKey="Weber, James L" sort="Weber, James L" uniqKey="Weber J" first="James L." last="Weber">James L. Weber</name><name sortKey="Wells, Dan E" sort="Wells, Dan E" uniqKey="Wells D" first="Dan E." last="Wells">Dan E. Wells</name><name sortKey="Wood, Stephen" sort="Wood, Stephen" uniqKey="Wood S" first="Stephen" last="Wood">Stephen Wood</name><name sortKey="Yu, Chang En" sort="Yu, Chang En" uniqKey="Yu C" first="Chang-En" last="Yu">Chang-En Yu</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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